mouse cd47 orf cdna  (Sino Biological)

Journal: Nanotechnology, Science and Applications

Article Title: EGF Receptor-Targeting Cancer Therapy Using CD47-Engineered Cell-Derived Nanoplatforms

doi: 10.2147/NSA.S352038

Figure Lengend Snippet: Preparation and characterization of CD47+ iCDNs-DOX. ( A ) The process of CD47 + iCDNs-DOX preparation is illustrated. ( B ) The prepared CD47 + CDNs, CD47 + CDNs-DOX, CD47 + iCDNs, and CD47 + iCDNs-DOX (from left to right) were negatively stained with 2% uranyl acetate and then examined with a transmission electron microscope (×80 (K)). ( C ) The size and surface charge of the nanoparticles were analyzed three times with a ζ-sizer. Each diameter (nm) and zeta-potential represents the mean ± S.D. for three independent experiments. ( D ) The change of CD47 + CDN size during storage at 4℃ was monitored for 10 days. Each error bar represents the mean ± S.D. for three independent experiments.

Article Snippet: The mouse CD47 ORF cDNA clone expression plasmid, C-GFPSpark tag (pCD47-GFP), was purchased from Sino Biological Inc. (Beijing, China).

Techniques: Staining, Transmission Assay, Microscopy, Zeta Potential Analyzer

Journal: Nanotechnology, Science and Applications

Article Title: EGF Receptor-Targeting Cancer Therapy Using CD47-Engineered Cell-Derived Nanoplatforms

doi: 10.2147/NSA.S352038

Figure Lengend Snippet: Interaction of CDNs with macrophages. ( A ) The expression of CD47-GFP fusion protein on the cell surface was identified using a confocal microscope (×800). ( B ) Western blotting of transfected cell lysates and CDNs verified the CD47 presence. ( C ) RAW264.7 cells were treated with DiD-labeled CD47 − CDNs or CD47 + CDNs for 1 or 4 h, and then subjected to flow cytometry. ( D ) The macrophages incubated with CDNs for 4 h were also examined with a confocal microscope (×800). ***, p<0.001 vs cells treated with CDNs. Each error bar represents the mean ± S.D. for three independent experiments.

Article Snippet: The mouse CD47 ORF cDNA clone expression plasmid, C-GFPSpark tag (pCD47-GFP), was purchased from Sino Biological Inc. (Beijing, China).

Techniques: Expressing, Microscopy, Western Blot, Transfection, Labeling, Flow Cytometry, Incubation

Journal: Nanotechnology, Science and Applications

Article Title: EGF Receptor-Targeting Cancer Therapy Using CD47-Engineered Cell-Derived Nanoplatforms

doi: 10.2147/NSA.S352038

Figure Lengend Snippet: EGFR-specific cell biding of CD47+ iCDNs and tumor targeting and cytotoxicity of CD47+ iCDNs-DOX. ( A ) MDA-MB-231 and MDA-MB-453 cells were treated with Alexa fluor 488-labeled CD47 + iCDNs and then subjected to FACS analysis (upper). ( B ) MDA-MB-231 and MDA-MB-453 cells were treated with CD47 + iCDNs-DOX and stained with DAPI. The treated cells were examined with a confocal microscope at various time points. PMT, photomultiplier tube. ( C ) The tumor cells were also treated with various concentrations of DOX in a free form, CD47 + CDNs, or CD47 + iCDNs for 48 h, and the cell viability was quantified using the CCK-8 assay. The mean fluorescence of the treated cells was calculated (n=5) (bottom). ***, p<0.001 vs cells treated with CDNs. Each error bar represents the mean ± S.D. for three separate experiments.

Article Snippet: The mouse CD47 ORF cDNA clone expression plasmid, C-GFPSpark tag (pCD47-GFP), was purchased from Sino Biological Inc. (Beijing, China).

Techniques: Labeling, Staining, Microscopy, CCK-8 Assay, Fluorescence

Journal: Nanotechnology, Science and Applications

Article Title: EGF Receptor-Targeting Cancer Therapy Using CD47-Engineered Cell-Derived Nanoplatforms

doi: 10.2147/NSA.S352038

Figure Lengend Snippet: Biodistribution of CD47+ iCDNs in MDA-MB-231-xenografted mice. ( A ) The mice bearing MDA-MB-231 tumors were intravenously injected with DiO-labeled CD47 + CDNs or CD47 + iCDNs. ( B ) The whole-body fluorescence images were taken with an in vivo imaging system Then, major organs were removed, and the fluorescence images of the organs were also taken. ( C ) The relative fluorescence intensities of the organs at each time point were calculated by dividing the measured fluorescence intensity by area. ( D ) Tumor-to-liver ratios of CD47 + CDNs and CD47 + iCDNs were compared to each other at the same time point. Each error bar represents the mean ± S.D. of three measurements.

Article Snippet: The mouse CD47 ORF cDNA clone expression plasmid, C-GFPSpark tag (pCD47-GFP), was purchased from Sino Biological Inc. (Beijing, China).

Techniques: Injection, Labeling, Fluorescence, In Vivo Imaging

Journal: Nanotechnology, Science and Applications

Article Title: EGF Receptor-Targeting Cancer Therapy Using CD47-Engineered Cell-Derived Nanoplatforms

doi: 10.2147/NSA.S352038

Figure Lengend Snippet: Suppression of in vivo tumor growth by a series of CDNs-DOX. ( A ) MDA-MB-231 cells were subcutaneously implanted into mice, which were then intravenously injected with saline, free DOX, CD47 − CDNs-DOX, CD47 + CDNs-DOX, or CD47 + iCDNs-DOX at days 1, 4, and 7. ( B ) Tumor volumes were measured once every 3–4 days. ( C and D ) On day 39, the tumors were excised and weighed. ( E ) The body weights of the mice were also measured on the measurement days. Each error bar represents the mean ± S.D. (n=4). *, p<0.05 and **, p<0.01 vs CD47 − CDNs-DOX-injected group.

Article Snippet: The mouse CD47 ORF cDNA clone expression plasmid, C-GFPSpark tag (pCD47-GFP), was purchased from Sino Biological Inc. (Beijing, China).

Techniques: In Vivo, Injection, Saline

Journal: Nanotechnology, Science and Applications

Article Title: EGF Receptor-Targeting Cancer Therapy Using CD47-Engineered Cell-Derived Nanoplatforms

doi: 10.2147/NSA.S352038

Figure Lengend Snippet: Histopathology of the major organs of mice treated with a series of CDNs-DOX. Tumors and major organs including the heart, kidneys, liver, and lungs were removed and dissected on day 39 post-injection of saline, free DOX, CD47 − CDNs-DOX, CD47 + CDNs-DOX, or CD47 + iCDNs-DOX. The organs were embedded in paraffin, sliced, and then hematoxylin and eosin (H&E)-stained. The black dotted lines in the tumor pictures show the necrosis area (bright pink).

Article Snippet: The mouse CD47 ORF cDNA clone expression plasmid, C-GFPSpark tag (pCD47-GFP), was purchased from Sino Biological Inc. (Beijing, China).

Techniques: Histopathology, Injection, Saline, Staining